GLC t G6P

Glycolysis

Glucose + ATP <-(Hexokinase)->>> Glucose 6-phosphate + ADP + H

Hexokinase

Fundamentals:

• Glucose is transported by specific transport proteins into the cell
• Glucose is phosphorylated by ATP to form Glucose 6-phosphate
  • Phosphorylation changes polarity of glucose
    • keeps the molecule inside the cell, does now allow the passage out of the membrane
  • Phosphoryl groups destabilize glucose
    • sets up an isomerization of the 6C structure

Specifics

• Hexokinase has two lobes that move toward each other
• Removal of water form the active site enhances the specificity of the enzyme
• Binding site could attack the gamma phosphoryl group of ATP forming ADP and Pi