3PG to 2PG

Glycolysis - Gluconeogenesis
Phosphoglycerate Mutase

3-phosphoglycerate <-(Phosphoglycerate Mutase)-> 2-phosphoglycerate

Glycolysis

2-phosphoglycerate <-Phosphoglycerate Mutase)-> 3-phosphoglycerate

Gluconeogenesis

Mechanism:

General

• Phosphorylation and dephosphorylation
  • NOT an intermolecular attack of the alcohol with the phosphate
    • WHY? - educated guesses
      • Enzyme holds the molecue at a particular shape
      • Enzyme has an affinity = rate that revents intermolecular attack
      • Steric hindrance of the 2O(-)s means that there is an extra electron (6 total) repelling
        • Environment is slighlty basic pH of 7.3-ish so this allows the Os on the phoshate to remain deprotonated
        • To me since there is resonance, it seems like the steric hindrance is further intensified by the "swatting" of the O atom as it converts from a singe tetrahedral configuration to a planar carbonyl.
          • Imagine that the three Os are all in resonance periodically "swatting" away the chances for an intermolecular attack
          • MAYBE the concept of a feris wheel and mini golf put puts
            • each O as it swats away the chance of OH intermolecular attack